OmegaQuant Methodological Considerations

The Omega-3 Index is the EPA+DHA content of red blood cells (RBCs) expressed as a percent of total identified RBC fatty acids. It is measured using a proprietary methodology developed over several years of research. OmegaQuant, LLC is a CLIA-certified lab (#43D1105229).

The assays we perform at OmegaQuant Analytics, LLC have been validated and standardized. The details have not been published as they are proprietary. However, relevant data are available upon request from OmegaQuant for potential research clients.

    Assays for concentrations of EPA, DHA and AA in both total plasma/serum and in the non-esterified (i.e., unbound) fraction have been validated per FDA Guidelines for Industry. This includes data on

    • Linearity
    • Precision (CV’s are between 5% and 8% for all 3 fatty acids)
    • Sensitivity (lower limits of detection)
    • Specificity
    • Freeze-thaw stability
    • Short-term (plasma stored 1 week a room temperature or 4 weeks at -80°C)

    Our red blood cell fatty acid assay has been evaluated for

    • Linearity
    • Precision (CV’s are between 5% and 8% for all 3 fatty acids)
    • Sensitivity (lower limits of detection)
    • Specificity
    • Freeze-thaw stability
    • Short-term (plasma stored 1 week a room temperature or 4 weeks at -80°C)
    • Biological stability
    • Effects of fasting/fed state
    • Stability over time under several storage conditions (-80°C, -20°C, 4°C and room temperature)
    • Stability of both plasma and RBC fatty acids after storage in EDTA blood tubes at both room temperature and at 4°C
    • Accuracy of extrapolation to the Omega-3 Index from dried blood spot EPA+DHA
    • Preservation of samples dried on filter paper using our proprietary antioxidant mixture OxyStop®
    • Effects of exposure of dried and liquid samples to high heat conditions

    The other sample types we work with [liquid breast milk, dried milk spots, whole blood, dried blood spots and plasma (composition and concentration)] have been evaluated for stability over time under several storage conditions (-80°C, -20°C, 4°C and room temperature).

    We have compared Omega-3 Index results from 4 other laboratories to those from OmegaQuant for the analysis of a single blood sample.

    We have performed several studies to define the correlations between EPA+DHA measured in RBC and dried blood spots with EPA+DHA in plasma (as a % of total fatty acids and as a concentration) and in plasma phospholipids.

    We have shown that the fatty acid composition of RBCs and of plasma can be derived from frozen whole blood samples by separation of membranes from plasma lipids in an ultracentrifuge.

    Additional validation studies have been conducted; please contact info@omegaquant.com for more information.